By Tissue Brain - Spinal cord (cervical c-1) Track Settings
Assembly: Human Feb. 2009 (GRCh37/hg19)
Description
The
NIH Genotype-Tissue Expression (GTEX) project
was created to establish a sample and data resource for studies on the relationship between
genetic variation and gene expression in multiple human tissues.
This track hub shows RNA-seq read coverage signal graphs of GTEx samples in the
GTEx midpoint milestone data release (V6, October 2015).
A total of 7572 tracks representing samples from 570
adult post-mortem individuals are included.
Display Conventions
The individual sample tracks are organized by gender (Female Donors, Male Donors) and age,
in 6 tracks, where samples can be selected from a donor vs. tissue matrix.
Female 20-49 yrs
Female 50-59 yrs
Female 60-69 yrs
Male 20-49 yrs
Male 50-59 yrs
Male 60-69 yrs
An alternative organization is provided by the 'By Tissues' supertrack set, which consists of
a track containing all samples for each of the 53 tissues, with a selection matrix of
donor gender and age.
Methods
Tissue samples were obtained using the GTEx standard operating procedures for informed consent
and tissue collection, in conjunction with the
National Cancer Institute Biorepositories and Biospecimen.
All tissue specimens were reviewed by pathologists to characterize and
verify organ source.
Images from stained tissue samples can be viewed via the
NCI histopathology viewer.
The Qiagen PAXgene non-formalin tissue preservation product was used to stabilize
tissue specimens without cross-linking biomolecules.
The Illumina TruSeq protocol was used to create an unstranded polyA+ library sequenced
on the Illumina HiSeq 2000 platform to produce 76-bp paired end reads at a depth
averaging 50M aligned reads per sample.
Sequence reads for this track were aligned to the hg38/GRCh38 human genome using STAR2
assisted by the GENCODE v24 transcriptome definition. The read coverage signal graphs
were produced using the --outWig option of STAR2. Read mapping was performed at UCSC by the
Computational Genomics lab, using the Toil pipeline. The resulting bedGraph
files were converted to bigWig format for display. The bedGraph files were coordinate
converted to the hg19/GRCh37 assembly via the UCSC liftOver tool.
Subject and Sample Characteristics
The scientific goal of the GTEx project required that the donors and their biospecimen
present with no evidence of disease.
The tissue types collected were chosen based on their clinical significance, logistical
feasibility and their relevance to the scientific goal of the project and the
research community.
Postmortem samples were collected from non-diseased donors with ages ranging from 20 to 79. 34.4% of donors were female and 65.6% male.
Additional summary plots of GTEx sample characteristics are available at the
GTEx Portal Tissue Summary page.
Credits
Samples were collected by the GTEx Consortium.
RNA-seq was performed by the GTEx Laboratory, Data Analysis and Coordinating Center
(LDACC) at the Broad Institute.
John Vivian, Melissa Cline, and Benedict Paten of the UCSC Computational Genomics lab were
responsible for the sequence read mapping and signal file generation used to produce this hub.
Kate Rosenbloom and Parisa Nejad of the UCSC Genome Browser group were responsible for
data file post-processing and track hub configuration.
US Server
